Engineering wheat (Triticum aestivum) Essay Example

Engineering wheat (Triticum aestivum) Essay Describe how you would place the wheat asparagine synthetase cistrons based on homology to cistrons from other species, clone the complementary DNA and confirm cistron map. I would foremost obtain the sequences for the Arabidopsis thaliana and rice ( Oryza sativa ) asparagine synthetase ( AS ) cistrons from Pubmed, and compare this to the wheat genome sequence utilizing BLAST online. This can be seen for the wheat AS cistrons: TaASN1 and TaASN2 and putative homologues have been described on Homologene on the Pubmed ( NCBI ) web site. Homologues include the ASN2 A ; 3 cistrons in A.thaliana and the theoretical Os06g0265000 cistron in Oryza sativa. A survey performed by Wang H et Al to insulate the wheat ( Triticum aestivum ) TaASN1 and TaASN2 cistrons, compared them for homology to AS cistrons of other beings, they found that the glutamine binding sites and Class-II glutamine amidotransferase ( Glutaminase ) sphere were conserved. We will write a custom essay sample on Engineering wheat (Triticum aestivum) specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on Engineering wheat (Triticum aestivum) specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on Engineering wheat (Triticum aestivum) specifically for you FOR ONLY $16.38 $13.9/page Hire Writer Hence we could plan primers from the parts of homology conserved in AS cistrons, cognizing that there is a good opportunity they will be successful for usage in cloning the complementary DNA of the wheat AS cistrons. Genomic DNA could be extracted from wheat by homogenizing wheat tissue, as described by M A ; oslash ; ller et Al in which they did this for the liliopsid, barley. The method comprised of homogenising works tissue by puting about 200 A ; micro ; g into a 2.2-ml plastic tubing with two cylindrical beads, stop deading it in liquid N and crunching the tissue into a pulverization by vortexing at high velocities. Subsequent centrifugation and washing stairss would let the extraction of genomic wheat Deoxyribonucleic acid. Using the primers designed from the homologous parts of AS cistrons, we could so magnify a part of Deoxyribonucleic acid from this cistron via PCR to obtain a longer fragment of genomic DNA to plan better primers. The new primers can be used with messenger RNA extracted from wheat grain in the procedure of contrary written text PCR ( RT-PCR ) . This would bring forth cloned wheat complementary DNA at the AS cistrons TaASN1 and TaASN2. I could so infix the complementary DNA into a cloning vector, which contains the N-terminal fragment of the LacZ operon, leting testing cells when the vector is transformed. To corroborate cistron map, I would transform the complementary DNA vector incorporating the TaASN1 A ; 2 cistrons into E.coli, to do a complementary DNA library. The E.coli that I use will be knock outs for endogenous E.coli Asparagine Synthetase cistrons. They will besides miss the N-terminal fragment of the LacZ operon, similar to the M15 mutation of E.coli. E.coli, transformed successfully via electroporation, will incorporate a vector which has the N-terminal fragment of the LacZ operon, and the TaASN1 A ; 2 cistrons. E.coli would so be plated onto agarose home bases with media incorporating: X-Gal, IPTG ( a gratuitous inducer of the Lac operon ) and glutamate, but no asparagine. E.coli transformed successfully will be able to synthesize asparagines from glutatmate utilizing the TaASN1 A ; 2 cistrons on the vector, nevertheless untransformed cells will decease due to miss of asparagine. To farther guarantee that cells have been transformed successfully, the lasting cell settlements should be coloured bluish. The vector, incorporating the losing portion of the Lac operon, will let the transformed cells to change over the colorless X-Gal into a blue coloured substance. Hence bluish settlements that survive will be successfully transformed, and besides confirm that the wheat AS cistron s ( TaASN1 A ; 2 ) map is to synthesize asparagines from glutamate. The T-DNA concept seen in Figure 1 is designed to hush the TaASN1 A ; 2 cistrons in wheat grain. The Glu-1D-1 booster has been shown to be extremely endosperm particular to wheat grain by Lamacchia et Al and would therefore be ideal in pick in which to show the hushing concept in this experiment. This is because the T-DNA will merely be transcribed in wheat grain cells which have the ability to acknowledge this booster and transcribe Deoxyribonucleic acid after it. The silencing concept, shown as: ( AS2i, AS1i, I, AS1, AS2 ) , consists if the TaASN1 cistron and the TaASN2 cistron inserted as an upside-down repetition on either side of an intronic spacer sequence ( I ) . The ensuing concept, when transcribed specifically in wheat grain cells, will bring forth hairpin messenger RNA, due to the intronic spacer. The hairpin messenger RNA, which is dsRNA, will be recognised by the Dicer-RDE composite, which cleaves the messenger RNA into short fragments of 21-23bp in length. These fragments are referred to as short interfering RNA ( siRNA ) and are recognised by the RNAi silencing composite ( RISC ) , which binds to them and migrates to mRNA with complementary sequence. By adhering to and aiming the complementary messenger RNA for debasement the cistron look of the TaASN1 A ; 2 cistrons will be inhibited, because most of the messenger RNA transcribed from those cistrons will be degraded. Therefore this concept is structured to bring forth the effectual silencing of the TaASN1 A ; 2 cistrons. After the silencing concept, there is a expiration sequence which will hold written text, guaranting accurate written text of the hairpin messenger RNA. The Ubi1 booster is the corn ubiquitin booster and noncoding DNA, which will bring forth look of a cistron downstream to it ubiquitously throughout the wheat works. The cistron downstream of Ubi1 is shown as K res in Figure 1. It is a Kanamycin opposition cistron and is included in the T-DNA concept for usage as a selectable marker to test for successfully transformed hosts. There is another expiration sequence in the T-DNA, 3 A ; premier ; to the K res cistron, to guarantee that merely the K RESs cistron is ubiquitously transcribed in wheat. The T-DNA concept will be inserted into a vector as shown in Figure 2. It contains a Ampicillin opposition cistron, and beginnings of reproduction ( ORI ) that will let plasmid reproduction in both E.coli and A.tumefaciens. The vector will be is transformed into disarmed Agrobacterium tumefaciens, which have been modified to incorporate a helper plasmid and besides will hold had their Tumor-inducing plasmid removed. This assistant plasmid will incorporate the vir cistrons required to reassign T-DNA into the works and incorporate it into the works genome. The vir cistrons recognise the left and right boundary line repetitions, strike the T-DNA, reassign it into the works karyon, and intergrate it into the works s atomic Deoxyribonucleic acid. Therefore with this method the T-DNA concept will incorporate into cells of the wheat works, nevertheless the hushing concept will merely be translated in the wheat grain cells, as the Glu-1D-1 booster is merely active in these cells. Consequently the look of the TaASN1 A ; 2 cistrons will inhibited and the asparagine content of wheat should be reduced. Describe how you would transform the concept into wheat and isolate transgenic wheat workss. To transform the concept into wheat I have decided to utilize an Agrobacterium tumefaciens binary vector method of wheat transmutation. I chose to utilize this method over Bioloistics, because the reported transmutation frequence ( TF ) utilizing Agrobacterium is reported to be higher than that of Biolostics. Jones HD besides reported that utilizing immature blossomings as a wheat explant pick has shown good T-DNA look from Agrobacterium mediated transmutation. Hence I would transform immature blossomings from wheat, with my concept designed in Q2, utilizing and Agrobacterium mediated method of transmutation. I would transform the vector into E.coli via electroporation, to magnify the concept, choosing for successful transformants utilizing ampicillin opposition conferred by the vector. I would insulate the vector from the E.coli after elaboration, and transform the vector into Agrobacterium tumefaciens. The Agrobacterium will already hold been transformed with a assistant plasmid, which contains the Vir cistrons, which will let the transportation of T-DNA into the wheat. I would so submerse hurt immature blossomings into a suspension of the transformed A. tumefaciens. The workss would so be placed onto agarose civilization medium, incorporating Kantrex for choice of T-DNA incorporating transformed workss. Kanamycin will kill any workss which do non incorporate the vector, and the A. tumefaciens will decease due to miss of opines for foods. The media will besides incorporate the works endocrines auxin and cytokinin to excite root and shoot growing severally, and bring on callus formation. Hence merely transformed workss will organize a callosity, which can be regenerate in agar to organize plantlets and so grown in dirt to turn to the grownup wheat works. Describe how you would analyze the transgenic workss to prove for a nexus between look of the asparagine synthetase cistrons and acrylamide formation. First I would analyze the wheat grain utilizing RT-PCR to find the effects of the cistron hushing. I would homogenize both transgenic wheat grain and control wheat grain ( with a dummy vector incorporating no T-DNA ) and pull out the messenger RNA. Using the primers designed in portion 1 I would so magnify the messenger RNA produced by the TaASN1 A ; 2 cistrons. By running the merchandises on an agarose gel, the comparative look and activity of the cistrons can be measured and compared utilizing the comparative strengths of the sets in the gel. Transformed wheat grain should hold less messenger RNAs produced from the TaASN1 A ; 2 cistrons due to their suppression organize the silencing concept. I would besides analyze the transgenic wheat grain and toast made from the transgenic wheat grain, utilizing High Performance Liquid Chromatography ( HPLC ) to farther find the effects of the cistron hushing. It is of import to prove the acrylamide content of toasted staff of life, because acrylamide is formed from asparagine under high temperatures via the Maillard reaction. A low degree of acrylamide is the most of import consequence to obtain as it is the toxic substance in toast and will be ideal to take down. HPLC is a technique normally used in nutrient, cosmetic and environmental industries to observe the presence of compounds that could be harmful to the consumer. Compounds can be separated, identified and quantified utilizing this method, which basically consists of filtrating compounds through a column, based on assorted physical and chemical interactions. Rommens et al successfully used this method to find the asparagine content of murphies and the acrylamide content of Gallic french friess. Hence obtaining a low value of both asparagine and acrylamide, when compared to command wheat grain and control toast, will mean that the hushing concept transformed into wheat has successfully lowered the asparagine content and hence besides lowered the harmful acrylamide content of wheat. Reference List Wang H, Liu D, Sun J, Zhang A. Asparagine synthetase cistron TaASN1 from wheat is up-regulated by salt emphasis, osmotic emphasis and ABA. J Plant Physiol. 2005 Jan ; 162 ( 1 ) :81-9. M A ; oslash ; ller MG, Taylor C, Rasmussen SK, Holm PB. Molecular cloning and word picture of two cistrons encoding asparagine synthetase in barley ( Hordeum vulgare L. ) Biochim Biophys Acta. 2003 Jul 28 ; 1628 ( 2 ) :123-32. Lamacchia C, Shewry PR, Di Fonzo N, Forsyth JL, Harris N, Lazzeri PA, Napier JA, Halford NG, Barcelo P. Endosperm-specific activity of a storage protein cistron booster in transgenic wheat seed. J Exp Bot. 2001 Feb ; 52 ( 355 ) :243-50. Christensen AH. , Sharrock RA, Quail PH. Maize polyubiquitin cistrons construction, thermic disturbance of look and transcript splice, and booster activity following transportation to energids by electroporation. Plant Molecular Biology ( 1992 ) 18, 675-689 Jones HD. Wheat transmutation: current engineering and applications to grain development and composing. Journal of Cereal Science ( 2005 ) 41: 137-147 Rommens CM, Yan H, Swords K, Richael C, Ye J. Low-acrylamide French french friess and potato french friess. Plant Biotechnol J. 2008 Oct ; 6 ( 8 ) :843-53

Clever inventions. The possibilities of the mind

Clever inventions. The possibilities of the mind Useful inventions of all time If asked about the biggest invention of our time, people would probably name the Internet. It allows us to connect with friends around the world and establish connections we never knew existed. You want to talk to your favorite celebrity or find out what has been going on in Eastern Asia? - Google will help you! A hundred years ago, we could not imagine the luxury of interacting with people miles away from us. The credit goes to Lawrence Roberts, the inventor of the first Internet prototype. However, apart from the global communication system, what can we name as the greatest invention? You may argue about the usefulness of this or that device, but we can assure you they have all made a fair contribution to our routine. Where would the mankind be, if it was not for the phone or text messages we are used to exchanging whenever we want to share information? On top of that, we should include some of the things that were necessary for the development of the human race: The wheel. The wheel is truly one of the inventions of the humankind, the value of which cannot be overlooked today. The concept of the first wheel was developed in 3500 B.C., and it was a blessing for everyone. People had massive problems with transporting goods and moving them from one town to another, so we guess this discovery was by far one of the most expected in the history of human civilization. These days, wheels are used everywhere, and the history we know was shaped with the help of this tool. The compass. Do you remember Captain Jack’s compass that showed the thing you wanted most? Well, magic aside, sailors used to navigate by the stars. Suffice to say, it was not a very safe methodic, as it forced them to skip their craft on cloudy evenings or in the daytime. Before the invention of the first compass, captains were bound to obey the mere changes of the weather, and had to wait until the sky was clear again. The situation changed, however, when the compass we know today was invented. The secret to successful navigating is the magnetized needle, which points north. The printing press. As we know, very few people had access to books back in the Middle Ages. The printing press appeared as a revolutionary device and changed the way we perceive printing today. Johannes Gutenberg decided to alter the course of history by inventing a special machine, which pressed ink to the paper so that the paint remained on the surface for long. This is another invention that led to the development of the human race, the importance of which cannot be overstated. The printing devices we have today allow us to immerse in the world of literature and become educated individuals through reading. The telephone. The invention of this device presents a series of experiments, which all led to failure. Many scientists struggled to build an electronic device, which would allow people to communicate via wires. However, electronic transmission did not seem possible until Alexander Graham Bell took the initiative in his hands. He invented the phone in 1876, and was awarded a patent for his discovery that led to revolutionizing the world of communication. He had a lot of successors, who tried to improve the said gadget, and fortunately, phones as we know them today allow to transmit electronic signals at long distances. The light bulb. It is hard to imagine our daily lives without this device. Electricity was in poor state when Thomas Edison decided he would change the face of the world we know. Of course, we cannot attribute this success to his personality alone, as many people played a significant role in inventing the light bulb, but generally, the credit belongs to Edison. Prior to this, we depended on natural lightning and the light of the candles. That was romantic, in some ways, and uncomfortable as well, as people had to work during the day and finish their chores before the sun went down. Now, we can sit up all night and be sure the lightning is bright and comfortable, thanks to the invention that Thomas Edison proudly patented. Penicillin. The story behind the invention of Penicillin is awesome. In 1928 there was a Scottish scientist named Alexander Fleming, who conducted a series of experiments in his private laboratory. During one of the experiments, he left a lid on a dish with bacteria opened. Later, he discovered that bacteria were dead as the dish became contaminated with mold. Later, Fleming found out that the mold was called fungus Penicillium, and it was cleared and purified by scientists to turn it into a strong medication, which changed the course of science and was a big history turn in the first half of the 20th century.

Effect of Tax on the Selling of Apples in a Small Scale Assignment

Effect of Tax on the Selling of Apples in a Small Scale - Assignment Example This scenario takes two rounds or instances of selling and the supplier’s interest are the amount of profit. According to the data, in round 1 the supplier sold a bushel of apples at $10 and the second round at $19. The formula to calculate profit is: In the second set of data, session #1 has apples sold for every unit from 1 to 20. The indication is that the demand for the apples is high when there is no tax charged during the buying and selling of the apples. The demand for the apples in the experiment is less elastic than the supply. In the second session, the supplier bears the tax burden. In the experiment, the apple supplier still has a seller cost of $18 for a bushel of apples. Above the seller cost, the supplier must pay a sales tax of $15. However, if the seller does not sell any apples, he does not pay either seller cost or sales tax. From the record of prices and profits, the supplier never sold a bushel of apples in either round 1 or 2 showing a significant decline in the profits to zero when the supplier pays the tax. From the second data set, session #2 indicates a drop in the supply of the apples. In the first 14 units sold, the sellers supply pattern indicates some increase in volumes of the sales of apple. On the other hand, from 15 to 20 units, the seller never sold any apples due to the high costs from taxes. The tax burden shifts to buyers in the third session. The supplier of apples in the first set of data does not sell any apples due to drop in demand since the buyers deter from buying. In the second data set, the buyers could still afford the bushel of apples for up to 12 units sold. From 13 to 20 units, the supplier does not sell any apples. This experiment uses a method of comparative statistics to analyze the effect of the tax on demand and supply. The economic theory that comes into play in this scenario is the optimal tax theory. This theory  suggests the best way to affect taxation with minimal distortion of the demand and supply. According to this theory, the sales tax imposed on suppliers and buyers has the same effect; reducing the demand and supply.

Enron Corporation Scandal Research Paper Example | Topics and Well Written Essays - 2500 words

Enron Corporation Scandal - Research Paper Example The Enron Corporation was founded in the year 1985 and was one of the leading companies offering services in electricity, communications, natural gas and pulp and paper industries. The financial performance of Enron Corporation had also been uniquely impressive before its collapse in 2001 where its annual revenues showed an increase of $9 billion to over $ 100 billion from year 1995 to 2000. However at the end of year 2001 it was brought to attention and revealed that the company’s reported financial condition was sustained mainly because of the systematic, institutionalized and creative plan of accounting fraud. It has been reported by Thomas (2002), that the shares of Enron Corporation dropped from $90 per share in the mid of year 2000 to nearly less than $1 per share by the end of year 2001, this drastic decrease in the stock price of the company caused shareholders a loss of $11 billion. Thus Enron Corporation revised its financial statements for the past five years declar ing a loss of $586 million only. In the December of 2001, Enron fell to bankruptcy. Also with the revelation of Enron Scandal in 2001, Arthur Andersen, one of the biggest audit and accountancy partnerships also dissolved. Enron undoubtedly was the biggest audit failure but this company that was famous in the world, also became famous for its so sudden collapse (Li, 2010). The scandal of the Enron Corporation is one of the most notorious scandals of the American corporations. It is commonly believed by economists, historians and analysts that the case of Enron is undoubtedly a case of White Collar Crime. The term White Collar Crime is used as a phrase when various interrelated and overlapping areas of law including the misappropriation, securities law, fraud, bankruptcy, money laundering, financial frauds, corruption, tax frauds, cybercrime, government frauds, bribery etc happen or occur. Hence white collar crime is not a discipline that is autonomous rather it is interdisciplinary t hat tends to combine individuals and professionals from various professions and disciplines which may include auditors, accountants, law enforcement, investigators and attorneys (Â  Gill & Scott, 2008). In view of the white collar crime Enron Corporation which was a big name in the corporate sector and was of one of those corporations that were seen to be practicing price stability, merchant bank selling and hedging of funds. However the later discovery of this information that Enron was nothing less than a giant fraud changed the course of it. The nature of fraud at the company was both classic as well as modern frauds. The charges of White Collar Crime include: 1. Involved in Ponzi Schemes The company was engaged in the Ponzi scheme, whereby the top executives of the company that were in charge of running the company became rich at the expense of its employees and the investors. This all was happening with the support of Arthur Anderson, an accounting firm – which further disclosed that the main business of Enron Corporation was selling of stocks, which was the main profit generating source for the company (Pontell). 2. Involved in Hype and Dump Manipulation Schemes Similarly Enron was also involved in the hype and dump manipulation schemes, whereby it greatly inflated its stock prices

Intellectual Property Law Essay Example | Topics and Well Written Essays - 2750 words - 1

Intellectual Property Law - Essay Example England has been slow in adopting these rights as it is perceived as clashing with the principle of Freedom of Expression. The English contend that intellectual property rights costs public good. Advances in digital technology have shaken the grounds on which intellectual property stand. This has made people to focus more attention on coming up with ways of ensuring freedom of expression that would enhance the public domain whilst rewarding creativity. Image rights promote invention and creation that promote quasi monopoly or monopoly rights so as to accord creativity the credit it deserves. Celebrities in music, sports and media world have gained profound significance in the contemporary world. Product sellers and advertisers are increasing approaching celebrities for work as they want to be associated to this fame. Competitive brand ambassadors help popularize products and increase sales. The financial benefits that accompany the status of such celebrities implore the need to have a mechanism of protecting celebrities. Red Croz would need to know that there is the need to balance between their public and private lives. They are prone to misuse and exploitation, even if indirectly, by those who live and work around them. Image rights promote the right to privacy hence upholding the human dignity of such celebrities (Passman 2012, p.2). ... A device mark logo involves an image, for example, that of an animal. Red Croz’s logo is a stylized mark. The most common way to protect a logo is using trademark. Even so, this depends on how the mark will be used. When using a logo as a combined mark, it is enough to use a single combined registration. In instances where the elements in a logo are used separately, each element is registered separately and also in combination (Kemp & Hill, 2005). Copyright registration can also be used to protect a logo. In the United Kingdom, the creator of a work gets its copyright automatically. A contract is needed to change ownership of such a work. In the United States of America, one needs to register copyright of a work through Library of Congress. Proof is needed to stop another individual or business from using another’s logo. This is because the other business or individual could claim that their logo was stolen or copied. Establishing upfront solid evidence is important if logo copyrighted through registration. This evidence would be needed in a court of law when challenging logo infringement (Passman 2012, p.4). A logo can also be registered as a design or a trademark. Registering a logo as a design is more powerful than registering it as a copyright. Registering a logo a design helps deter logo copying. This registration allows a business or individual to stop others from using logos that are too close to theirs even if they did not copy them. In the United Kingdom and in the European Union has an allowance for design registration of logos. The registration is renewable after every five years for a period of twenty five years. Design registration of a logo is given a period of one year after which

The Term Of Nakakainis In The Philippines English Language Essay

The Term Of Nakakainis In The Philippines English Language Essay Have you encountered a time when you are feeling really irritated or annoyed that you would just like to punch or slap a pesky person? When you are being disturbed by an utterly foolish person who asks nonsense questions? Or when you did not get to watch your favorite soap opera? These situations make you say NAKAKAINIS!!, right? But Filipino youth these days barely use the original meaning of nakakainis irritated, annoyed and troubled (Nakakainis in English, Yahoo Answers. June 2010). Filipino youth would say NAKAKAINIS if they find things which are interesting like after watching this video (Ayos sa Trip si Manong, You Tube . June 2010), seeing this very cute picture (Baby Cheeks, Flickr [picture file]. November 2005) or cracking Filipino funny jokes like Q: bakit bawal maligo ng sabay-sabay ang mga kalbo sa Jacuzzi? A: Kasi, magmu-mukha silang pinipritong squid ball!! They would say NAKAKAINIS!! even if they are not feeling irritated or annoyed. This is the NAKAKAINIS of our youth . We can also say that it is the modern nakakinis for the youth. NAKAKAINIS is a form of expression that is used by Filipino youth. They use it when they see interesting pictures, videos, mannerisms or stuffed toys. The word nakakainis has a prefix nakaka- and a root word inis. In Filipino, the prefix or unlaping naka- is used when the word will be used as a conjugate in the sentence. If the essay or sentence must be written in formal, the conjugate should be written having its prefix nakaka- first, and the root word itself. Example: nakakabaliw, nakakamatay, nakakagalit. If it is informal, the prefix naka- should be written fist, followed by the first syllable of the word, and the root word itself. Example: nakababaliw nakamamatay, nakagagalit. (Panlapi, Gintong Pamana: Aklat sa Wika 3. 2006) The word nakakainis has two different meanings the feeling of irritation or annoyance and the reaction of the showing of interest in a thing. Nakakainis, in English, means irritating or annoying. According to the definition of annoyance, if the feeling of irritations and annoyance mount up, our feeling outbursts and thus, the feeing of irritation and annoyance will be changed or result in anger or frustration.(Annoyance, Experience Festival. n.d.) It is an uncomfortable feeling that can be the reason of a sudden outburst of anger. Sometimes, if a person is really irritated or annoyed, he could not control his emotions and do something rush that would result to a big problem. People who doesnt have control in their feelings would shout or hurt the feelings of the person who irritates or annoys them. When we get angry because of irritation and annoyance, we should have presence of mind to prevent unwanted events to happen like fighting. The expression nakakainis, in a high-pitched tone, can mean the reaction after seeing interesting things. For example, a teenager sees you carrying a cute Crayola pillow and her reaction will surely be: Its so cute! Where did you buy that? Nakakainis! or I would also like to have one! Nakakainis ang pagka-cute nya! In my high school, my classmates used nakakainis as an expression whenever they find cute and interesting things. Making a person happy of satisfying a persons need is the same as pleasing them while annoyance or irritation is when somebody or something making you feel uneasy by continuously bothering you. Pleasing a person is not easy as annoying or irritating a person. We can contrast irritation or annoyance to pleasing a person. It takes time and effort to please somebody. Most of the time, people please others because they are scared of rejection. They are scared to be left alone and thus, making themselves always available even though they are busy. People-pleasers, those people who please others, feel that people are taking advantage of them because of their low self esteem. People-pleasers always suffer because of low esteem and they cant say no to people who ask them favors or requests. Ms. Carla Valencia stated in her blog that those people who like to please others are scared of people rejecting them, preventing problems between other people, problem on always degrading themselves beca use they are not confident enough on their abilities, skills or knowledge. (Low Self Esteem and Pleasing People, More Self Esteem. n.d.) Stress can be the result of being irritated and annoyed always. As stated earlier, being irritated or annoyed can lead to emotions such as anger and frustration. We all know that stress has a great effect in our lives. It makes us age prematurely, can lead us to have psychosomatic illness and greatly affect our job. Stress can be more of a factor in determining your physical age than the number of candles you blow out each year. (#7 Effects of Stress, About. November 2007) Because of stress, a person can look older than his present age. A good example for this one is the commercial from the StressTabs. In the commercial, Giselle Sanchez, the host of the bar, thought that a looking sick and old girl was already in her 50s so she called her as lola. Then the boyfriend of the girl told Giselle Sanchez that she is his girlfriend and she looks like that because she is stressed and tired from work. But the girl, after taking StressTabs, looked like she transformed into a pretty, fine, youn g-looking girl. This commercial is telling us that being stress always would ruin our beautiful face and will only look older. According to the number five of the effects of stress, it is stated that thinking negative things and being emotionally stressed results in psychosomatic illness. We should be worrying about it because it can be treated like in any other kind of sickness. (#5 Effects of Stress, About. November 2007) People can cure themselves by attending Yoga classes. By doing Yoga, stress will go away and it can refrain us from getting or acquiring psychosomatic illnesses. People should always think positive things to also prevent from being stressed and sick. We, people, need to have energy to work. But, if we are always stressed, we cant do our job well. People need to rest to have energy. The Filipino expression kakagigil or a intense emotion, urge or feeling that a person gets over with something that he/she thinks is totally wonderful and together describes carefully how a person responds or want to respond (Getting Gigil with It, Culture Fried. March 2010), can be compared to kakainis. NAKAKAINIS is a form of expression that is used by Filipino youth. They use it when they see interesting pictures, videos, mannerisms or stuffed toys. The expressions that we daily use reflects on our attitudes. Most expressions are slang and vulgar words. We should be wary of people because they will base their first impressions on other people by just hearing their expressions. Especially here in the Philippines, most expressions used are curses, slang and other vulgar words. We should be careful in every word we say. Expressions are like any other words we encounter. As we all know, words are dynamic. Thus, the expressions we daily use could also evolve its meaning in years to come.

The big day :: essays papers

The big day I really hated the sound of that alarm clock, that piercing, irritating repeated beeping. After a second or two I slowly started realizing that it was not just another day, it was the day. I felt the movement in the bed as she reached for the clock and then the beeping stopped allowing me to slip back towards tranquillity again. "Love you," I whispered. "Excuse me, you were saying?" she said sarcastically. "You heard me," I said a little louder yet trying not to strain my tired voice. "I didn't say I didn't fucking hear you, I asked you what you said. Repeat it, louder. And try opening your eyes this time." After a moment of contemplating the situation I forced myself to make an effort and sat up, looking at her. Everything about her was beautiful even in the morning. The way that curly almost black hair just touched her shoulders. The casual pose she held sitting on the edge of the bed in that worn Lakers T-shirt. I took her hand and pulled her a little closer admiring her mischievous smile. She wanted to say something but she waited for me to speak first. I kissed her hand repeatedly and looking into her dark brown eyes I said slowly overenunciating each word: "I...love...you." Her smile got wider as she replied. "You better. Don't you dare flake on me now." She lay down beside me and kissed me gently wrapping her arms around me. I slid my hands inside her T-shirt running them up and down her back and I said: "We really don't have time for this." "Absolutely not. How about the shower?" And the shower it was, taking far too much of the time we did not have. I stood shaving when she asked from the bedroom: "Denny's or Jack in the box?" "Which one is the most romantic?" "Breakfast in bed!" "OK, you got me. How about Big Bob's in Burbank?" "You call Big Bob's romantic?" "We're not making love there, we're eating." "It's a drive, I'm hungry. I wanna eat now." I looked at my reflection in the mirror, undergoing one of those mini crises wondering if it was really me standing there. Was that really what I looked like, who I was? That was my face, my body, and I would spend the rest of my life confined within it. Even though I was quite familiar with my own image, he seemed a little like a stranger. "Well?" she said, and I suddenly snapped back into the present. "Er, is Jack in the box drive-through fine?" "Perfect." "Perfect," I thought.

History of Auguste Escoffier and Marie Careme Essay

Auguste Escoffier was born in Villeneuve-Loubet,the Provence region of France in October 28, 1846. When he turned 13, his father took him to Nice where he apprenticed at a restaurant owned by his uncle, thus beginning the illustrious career that he enjoyed for the next 62 years. His culinary career took him many places, from the early years at the fashionable Le Petit Moulin Rouge and several other restaurants in Paris, to Monte Carlo, Switzerland, and London. In 1870, when the Franco-Prussian War began, Escoffier was called to duty in the army where he served as Chef de Cuisine. It was during this period that he came to consider the need for tinned foods and was thus the first chef to undertake in-depth study of techniques for canning and preserving meats and vegetables. After returning to civilian life, Escoffier resumed his career in several Parisian restaurants where he steadily moved up the ladder of success. During his time at the Carlton, Escoffier developed a superior reputation for haute cuisine. While at the Savoy, Escoffier created one of his most famous recipes, Peach Melba, in honor of the Austrian singer Nellie Melba who was a guest at the hotel. Three of Escoffier’s most noted career achievements are revolutionizing and modernizing the menu, the art of cooking and the organization of the professional kitchen. Escoffier simplified the menu as it had been, writing the dishes down in the order in which they would be served (Service à   la Russe). He also developed the first à   la Carte menu. He simplified the art of cooking by getting rid of ostentatious food displays and elaborate garnishes and by reducing the number of courses served. He also emphasized the use of seasonal foods and lighter sauces. Escoffier also simplified professional kitchen organization, as he integrated it into a single unit from its previously individualized sections that operated autonomously and often created great wasted and duplication of labor. Throughout his career, Escoffier wrote a number of books, many of which continue to be considered important today. Some of his best-known works include Le Guide Culinaire (1903), Le Livre des Menus (1912) and Ma Cuisine (1934). As well as making changes in the culinary world, Escoffier undertook several philanthropic endeavors including the organization of programs to feed the hungry and programs to financially assist retired chefs. Escoffier received several honors in his lifetime. The French government recognized Escoffier in 1920 by making him a Chevalier of the Legion d’ Honneur, and later an Officer in 1928. The honors due Escoffier can be summed up by a quote from Germany’s Kaiser Wilhelm II when he told Escoffier, â€Å"I am the Emperor of Germany, but you are the emperor of chefs.† With his wife, Delphine Daffis, Escoffier retired to Monte Carlo in 1921, there he died on February 12, 1935. Marie Antoine Carà ªme, â€Å"Chef of kings and the king of Chefs.† In March 1811, Napoleon and his new wife, Marie Louise, welcomed the birth of a boy, the longed for male heir needed to carry the Bonaparte line forward. A grand feast was ordered to celebrate the christening of the young â€Å"King of Rome.† Only a year earlier, a young pastry chef named Marie-Antoine Carà ªme had dazzled the court with a still-talked about wedding cake. For the christening he would out-do himself again. Using spun sugar, confectioner’s paste, cream, and meringues all dyed in varying shades of blue, rose, and gold, Carà ªme created a magnificent replica of a Venetian gondola. He was abandoned in Paris at the age of 8 and began working as a kitchen boy in a Parisian steakhouse. At the age of 14 he was apprenticed to Sylvain Bailly, a famous patissier in Paris. Carà ªme codified the four primary families of French sauces that form the basis of classic French cooking to this day–espagnole, và ©loutà ©, allemande, and bà ©chamel. Thanks to Carà ªme’s books, French chefs working at home and abroad had a basic, shared vocabulary to refer to in their cooking. Marie-Antoine Careme is famed for being the inventor of classical cuisine. Careme crafted pieces for Parisian high society, including Napoleon. A French diplomat and gourmand, Charles Maurice de Talleyrand-Perigord set Careme a test, to produce a year’s worth of menu only using seasonal produce. Careme passed and Talleyrand adopted him into his kitchens. After the fall of Napoleon, Careme moved to London in 1815 and he worked as a chef de cuisine for George IV. He left London 3 years later as he found the climate depressing and felt English chefs treated him badly due to the celebrity attention he received. With the money he made as a freelance chef, Carà ªme opened his own pà ¢tisserieon the Rue de la Paix during the winter of 1803-04. Its windows regularly showcased his pià ¨ce montà ©es prompting travel guides to make it a recommended stop. He created these decorative centrepieces out of materials such as nougat, marzipan, sugar and pastry. Careme was inspired by architectural history and modelled many of his creations on temples, pyramids and ancient ruins. Some of his most famous creations include Gros Nougats, Grosses Meringues, Croquants (made with almonds and honey) and solilemmes (a bun like cake.) Contributions: The dessert Charlotte russe was invented by Marie Antoine Carà ªme who named it in honor of his Russian employer Czar Alexander I. Russe being the French word for â€Å"Russian†. Careme is also credited with inventing the French classic desert Napoleon Cake (Mille Feuille) while working as Napoleon’s chef. Napoleon like to eat Mille Feuille with strawberry favor, so it was named Napoleon cake. It has various flavours, from chocholate, strawbery, mango to berry. Careme was also interested in Architecture and applied it to dessert with is very impressive pieces montees and other creations that fascinated his contemporaries. (See pictures below) My Comment: First of all, I would like to thank both Auguste and Careme’s effort for what they have done in the food industry especially in the culinary field. Because of them we can now use or apply the things and knowledge that they had contributed to us in the late 18th century. Without them just imagine what a normal Restaurant kitchen would be without Escoffier’s Kitchen Brigade System? I guess it would be a total chaos and what about Careme’s four Mother sauces and his architectural designs in desserts? Just think what would be like in many special occasions especially in weddings? Without these ideas nothing good will happen to us. I actually admire them because at a young age they manage to be determined in many obstacles in life, they strive for their dream of becoming a real someone that led them to be famous in the food industry. What if I or we can do the same things that they’d done? Maybe someday we can be someone just like them in the future.

High school Confidential Essay

Some people say that high school is the most crucial part of a student’s life but others they say that it is the happiest chapter of a student’s life. There are a lot of things that a teenager wants to explore and it starts when one reaches high school; curiosity starts in this part of a person’s life. But did it ever occur to your minds what happens in a typical and an atypical high school class? Have you ever pictured in your minds what is it like being in a high school class? Have you ever wondered how the students’ relationship with their teachers is? What do the students do often? In this section â€Å"demented kindergarten†, of the book â€Å"High School Confidential† Iversen tackles about the relationship of an atypical high school students with their teacher. This chapter shows how the students get along in class and how they treat each other. It also talks about what goes on between the students. Through Charity and Vic’s relationship as classmates, as shown in the section demented kindergarten, Iversen shows that respect was lacking among the students. There is no good relationship between the two students; it employs that there was no respect for women. In this section, Iversen also shows the domineering nature of man over women. In Charity and Vic’s relationship it was shown that Vic does not have respect over Charity as a woman. Although Vic and Charity were classmates and charity was a girl Vic did not bother to talk to her in a disrespectful manner; it is not lawful to talk to women in an odd way because it degrades women’s confidence and dignity. When Vic said that he had a hung over, Charity was kind enough to tell him to take Advil for his headache, but instead of saying thank you to Charity Vic yelled at her and called her a slut. It was not proper for him to call Charity a slut because he is not in the position to scrutinize someone, most especially women. Disrespect and power over women was also portrayed through Vic and Sara. As Iversen had written in his book, Vic spun Sara’s chair until she can no longer take it and tumbled. Sara did not oppose Vic while he was spinning her chair; she just let him do what he wanted to do because Vic was more powerful than Sara; because Sara might have felt that she can not do anything to stop Vic because she was helpless. Although Vic knows that Sara would be helpless and might tumble if he would spin the chair faster he still managed to spin Sara’s chair without hesitation. In the social context of the book â€Å"High School Confidential, Iversen’s observation about how men disrespect women and the domineering nature of man is very common. Vic is powerful in the social structure of their school he was a bully. And because he is a man he is over empowering women, like Charity and Sara, around him. He is fund of dominating because he is a man; he wanted to show that he is more powerful than women. Men are domineering in nature and they seek superiority over women; they raise their manliness by doing things to make women look and feel helpless and less powerful. This nature of men is actually very visible in the society and in some cultures. Just like in Japan, men of Japan do typical things that a man does; earning money for the family and being the head of the family, they are not suppose to be seen in kitchen because it would be a disgrace for their manliness. While women of Japan are suppose to do only household chores, they are not entitled to do manly things. Some men show their manliness in other ways such as sadism, they tend to beat their wives because they wanted to show that they are the more powerful kind and that no one can oppose them most especially women, but some men do the beating habitually that’s why many government agencies help battered women. That is also why there are republic acts which tend to help women against violence. A single insult to women can be treated as violence against them; telling them that they are slut, whore and the likes. Cursing women and saying various words that might degrade them can be called violence against women. In the section â€Å"demented kindergarten† Iversen tells the domineering nature of men is a problem of our society. Everybody is not aware of this problem that is why Iversen made a way on how to communicate with the society through his book â€Å"High School Confidential†. Iversen wants others to know that in simple ways such as bullying a classmate can employ male domination. Iversen also exploited the disrespectfulness of men to women. Although the theme of the section is not that clear and visible unless understood, Iversen had still managed to write the book in the most convenient way he knows (Iversen)

Free Essays on Just Say No

â€Å"Just Say No† â€Å"Just Say No!† That is easy to say, but not so easy to do. In Stanley Milgram’s article, â€Å"The Perils of Obedience,† he discusses several similar experiments he conducted studying the human resistance to pressure and obedience. Contrasting his thoughts Diana Baumrind in her article, â€Å"Review of Stanley Milgram’s Experiments on Obedience,† critiques the comments of Stanley Milgram and his experiments. Milgram found that people would obey with the right situations, the right pressures and if they could pass off the blame to someone else. Baumrind contradicts Milgrams views on how he did things. Milgrams experiment was to see how far a person would go in hurting another human being. One man was brought in at random (the teacher) and another a person in on the experiment (the learner). The teacher was shown the student being strapped in to be electrocuted if he got something wrong. The teacher was informed on how to administer the tests and informed through out to continue the experiment no matter what. His first conclusions on the experiment were that in the right situations you could get most people to obey what they were told to do. The fact that the teacher had no previous or future connections with the learner was a good way of getting them to continue. Milgram compared the teacher to experimenter relationship to the relationship to Hitler and his soldiers. They listened because they new they had to or there would be consequences. The final way Milgram got people to listen and not worry about the consequences was allow them to think they could pass off the blame onto the experimenter and not have to take any responsibility. Milgram made the teacher think this way by telling them that the experimenter would take all the blame, and after the experiment was over the teacher was informed of everything. Milgram believed this would make them feel better about everything that took place ... Free Essays on Just Say No Free Essays on Just Say No â€Å"Just Say No† â€Å"Just Say No!† That is easy to say, but not so easy to do. In Stanley Milgram’s article, â€Å"The Perils of Obedience,† he discusses several similar experiments he conducted studying the human resistance to pressure and obedience. Contrasting his thoughts Diana Baumrind in her article, â€Å"Review of Stanley Milgram’s Experiments on Obedience,† critiques the comments of Stanley Milgram and his experiments. Milgram found that people would obey with the right situations, the right pressures and if they could pass off the blame to someone else. Baumrind contradicts Milgrams views on how he did things. Milgrams experiment was to see how far a person would go in hurting another human being. One man was brought in at random (the teacher) and another a person in on the experiment (the learner). The teacher was shown the student being strapped in to be electrocuted if he got something wrong. The teacher was informed on how to administer the tests and informed through out to continue the experiment no matter what. His first conclusions on the experiment were that in the right situations you could get most people to obey what they were told to do. The fact that the teacher had no previous or future connections with the learner was a good way of getting them to continue. Milgram compared the teacher to experimenter relationship to the relationship to Hitler and his soldiers. They listened because they new they had to or there would be consequences. The final way Milgram got people to listen and not worry about the consequences was allow them to think they could pass off the blame onto the experimenter and not have to take any responsibility. Milgram made the teacher think this way by telling them that the experimenter would take all the blame, and after the experiment was over the teacher was informed of everything. Milgram believed this would make them feel better about everything that took place ...

Wal-mart supermarket Essay Example | Topics and Well Written Essays - 1750 words

Wal-mart supermarket - Essay Example The final important consideration by the company is that they should prioritize business processes and procedures more than the technology at hand; technology should be incorporated on the basis of business needs rather than joining the race of being equipped with the latest technology. The formulation of this strategy helps the company to maintain the technological development and ensure that it is directed towards the acquisition of the company goals. The founders of Wal-Mart have always treasured their customers and employees by giving them the status of ‘associates’; Koselka (1992) stated that Wal-Mart benefits these stakeholders in the following manner; by giving better prices and quality to their customers and empowering their employees. Information has been an integral part in the growth of the organization. Relevant and latest information has been available to the company with the help of their information systems. 3. Point of Sales System K-mart was initially co nsidered to be the biggest competitor of Wal-Mart. When K-mart started improving their business processes with technology, Wal-Mart also realized the need and deployed point of sales system in their stores. This system identifies each product in the store on the basis of its unique number and gives the respective price of the product from a database that is maintained by the IT department. A receipt is automatically generated with the respective product numbers and prices. This information is added in the sales reports and also used in the process of reordering inventory when more quantity is needed. The hardware technology that is used to identify the products is the barcode scanner. Barcode scanner makes the data available in the database immediately...Wal-Mart adapted the barcode technology while being influenced by its competitor. The incorporation of technology in the company reflects their commitment to providing value to their customers and improving their service. The point of sales system that was equipped with barcode technology proved to benefit the company in many different aspects; from automatic generation of receipts to accurate entries of transactions, from immediate entry of records to providing latest data for analytical purposes. However, barcode technology was adopted by the respective organization when computer technology was not as common as it is today. The lack of skills of several employees in this regard caused a certain level of resistance towards the technological change. Effective measures were taken to get the system accepted by the employees. Wal-Mart also utilized information technology to develop effective networking links with their suppliers. This technique reduced their communication barriers and avoided instances of undersupply. Timely re-orders resulted in a constant supply of products in the store and results in effective service for the consumer. The suppliers networking with the retailers provide them with useful information about the buying behaviour of the consumer so that they can alter their products according to the consumer requirements. RFID is a developing technology that will prove to be even more effective than the barcode technology.

What were the violent techniques that (dictator of choice) used to Essay

What were the violent techniques that (dictator of choice) used to consolidate his power To what extent was he successful - Essay Example Finally, it is vital to identify the impact Fidel Castro dictatorship had on the people of Cuba. Fidel Castro a military general came into power 1959 after staging a coup de tat to over throw the then president of Cuba Fulgencio Batista (Thomas 16). Since then, Fidel Castro ruled Cuba for almost 50 years until he handed power to his brother in 2006. The fact that Fidel Castro held on to power for close to 50 years is evidence enough that he was a dictator (Thomas 19). One characteristic of a dictator is that he or she refuses to give up power for others to rule despite the fact that the county, which they rule is not a monarch system. Monarchies are the only form of government that rulers are allowed to rule for such a long time since power is mostly passed on to others only after death. Even after death power is left upon heirs or close members of the family. Evidence that Fidel Castro’s rule was dictatorship is the fact that his leadership was based on autocracy. This is a characteristic of dictator ship where rulers declare themselves sole rulers of a nation without any formal body to check on their power. This means that they can make decisions that affect the nation as they wish without consulting anybody especially the legislature. Another characteristic of dictators is that they have totalitarian governments. This is where they have firm control of the media controlling on what they broad cast. For example, Fidel Castro controlled the media to make sure that nothing negative about him was announced. The use of secret police and military units to spy on citizen is another form of a totalitarian government which is used by all dictators (Thomas 58). For example, Fidel Castro’s rule was characterized by such units to the point that people were even scared to discuss negative aspects of his government due to the fear of b eing arrested by these secret police and military